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A Fluorescence intensity at various time points following <t>iMSC</t> <t>Endostatin</t> recognition of tumors. B The endostatin-mCherry released by iMSC Endostatin at different time points was detected by a microplate reader. C The microplate reader was utilized to detect the total amount of endostatin-mCherry accumulated every four hours following full activation of iMSC Endostatin . D The amount of released endostatin by iMSC Endostatin was quantified using <t>ELISA</t> detection.
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A Fluorescence intensity at various time points following <t>iMSC</t> <t>Endostatin</t> recognition of tumors. B The endostatin-mCherry released by iMSC Endostatin at different time points was detected by a microplate reader. C The microplate reader was utilized to detect the total amount of endostatin-mCherry accumulated every four hours following full activation of iMSC Endostatin . D The amount of released endostatin by iMSC Endostatin was quantified using <t>ELISA</t> detection.
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A Fluorescence intensity at various time points following <t>iMSC</t> <t>Endostatin</t> recognition of tumors. B The endostatin-mCherry released by iMSC Endostatin at different time points was detected by a microplate reader. C The microplate reader was utilized to detect the total amount of endostatin-mCherry accumulated every four hours following full activation of iMSC Endostatin . D The amount of released endostatin by iMSC Endostatin was quantified using <t>ELISA</t> detection.
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A Fluorescence intensity at various time points following <t>iMSC</t> <t>Endostatin</t> recognition of tumors. B The endostatin-mCherry released by iMSC Endostatin at different time points was detected by a microplate reader. C The microplate reader was utilized to detect the total amount of endostatin-mCherry accumulated every four hours following full activation of iMSC Endostatin . D The amount of released endostatin by iMSC Endostatin was quantified using <t>ELISA</t> detection.
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A Fluorescence intensity at various time points following <t>iMSC</t> <t>Endostatin</t> recognition of tumors. B The endostatin-mCherry released by iMSC Endostatin at different time points was detected by a microplate reader. C The microplate reader was utilized to detect the total amount of endostatin-mCherry accumulated every four hours following full activation of iMSC Endostatin . D The amount of released endostatin by iMSC Endostatin was quantified using <t>ELISA</t> detection.
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A Fluorescence intensity at various time points following iMSC Endostatin recognition of tumors. B The endostatin-mCherry released by iMSC Endostatin at different time points was detected by a microplate reader. C The microplate reader was utilized to detect the total amount of endostatin-mCherry accumulated every four hours following full activation of iMSC Endostatin . D The amount of released endostatin by iMSC Endostatin was quantified using ELISA detection.

Journal: Cell Death & Disease

Article Title: Intelligent drugs based on notch protein remodeling: a defensive targeting strategy for tumor therapy

doi: 10.1038/s41419-024-07008-7

Figure Lengend Snippet: A Fluorescence intensity at various time points following iMSC Endostatin recognition of tumors. B The endostatin-mCherry released by iMSC Endostatin at different time points was detected by a microplate reader. C The microplate reader was utilized to detect the total amount of endostatin-mCherry accumulated every four hours following full activation of iMSC Endostatin . D The amount of released endostatin by iMSC Endostatin was quantified using ELISA detection.

Article Snippet: ELISA: MSCs or iMSC Endostatin will be co-cultured with Skov3-GFP for 48 h, and MSCs and iMSC Endostatin cells will be sorted by flow cytometry and cultured in a 24-well plate coated with fibronectin for 24 h. The content of endostatin will be detected using an ELISA detection kit (CSB-E07973h; CUSABIO).

Techniques: Fluorescence, Activation Assay, Enzyme-linked Immunosorbent Assay